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(2013) studied serum from 22 FM patients compared with 22 healthy female controls and identified significant alterations in purine metabolism, specifically decreased levels of adenosine, a precursor for ATP synthesis

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Cell culture Human immortalized epidermal cells (HaCaT cells, provided by the Shanxi Key Laboratory of Functional Protein) were cultured in DMEM medium containing high sugar (4.5 g/L), 10% fetal bovine serum, 100 U/mL penicillin, and 100 g/mL streptomycin