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Caspase-Glo reagent was added to the wells according to the manufacturers instructions and luminescent signal was measured on a SpectraMax M3 microplate reader (Molecular Devices)
Essentially, theres no strong evidence that B12 shots can harm youbut if youre not deficient, theres no guarantee theyll help you, either
As an essential vitamin, B12 is required for the body to work properly
[1] In practice, 1 mL (which provides the full 100 mg of B and B, etc.) is a common dose for general supplementation in deficient states, but clinicians may start with lower volumes for mild deficiencies or in smaller patients
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