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These sequences were then run through an R script (Supplementary Materials, bash_DADA2 + IDTAXA_workflow_Illumina-PairedEnd_V1V3.R) which trimmed reverse reads to 260 bp, removed forward reads exceeding a maximum expected error of three, and truncated reverse reads exceeding a maximum expected error of six

Echolaser Treatment: This technique uses laser energy delivered through thin needles to heat and destroy the excess prostate tissue

At the cellular level, ATF3 knockout enhanced SLC7A11/GPX4 expression and mitigated injury, identifying ATF3 as a core ferroptosis driver that regulates the System Xc /GSHGPX4 axis in I/R AKI